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fbw7 luciferase reporter plasmid  (Addgene inc)


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    Structured Review

    Addgene inc fbw7 luciferase reporter plasmid
    miR-27b modulated the expression of Snail by inhibiting its degradation ( A , B ) The mRNA level of Snail in CFs treated with AngII combined with miR-27i ( A ) or miR2-7b ( B ). ( C ) The effect of miR-27b on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( D ) Roles of miR-27b in Snail ubiquitination. ( E ) Characterization of the messenger RNA (mRNA) of <t>FBW7,</t> which depicted miR-27b binding site (BS) in its 3′-untranslated region (UTR). ( F ) The interaction of FBW7 and Snail in CFs. ( G ) The effect of FBW7 overexpression on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( H ) The effect of FBW7 overexpression on the ubiquitination of Snail. Arrow indicates the GFP-FBW7. Data were represented as mean ± SEM (n=4). N, p >0.05.
    Fbw7 Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fbw7+luciferase+reporter+plasmid/pRL-TK+3'FBXW7+UTR+(Plasmid+%2326649)/pmc06949061-188-4-14
    Average 90 stars, based on 1 article reviews
    fbw7 luciferase reporter plasmid - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway"

    Article Title: MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.102465

    miR-27b modulated the expression of Snail by inhibiting its degradation ( A , B ) The mRNA level of Snail in CFs treated with AngII combined with miR-27i ( A ) or miR2-7b ( B ). ( C ) The effect of miR-27b on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( D ) Roles of miR-27b in Snail ubiquitination. ( E ) Characterization of the messenger RNA (mRNA) of FBW7, which depicted miR-27b binding site (BS) in its 3′-untranslated region (UTR). ( F ) The interaction of FBW7 and Snail in CFs. ( G ) The effect of FBW7 overexpression on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( H ) The effect of FBW7 overexpression on the ubiquitination of Snail. Arrow indicates the GFP-FBW7. Data were represented as mean ± SEM (n=4). N, p >0.05.
    Figure Legend Snippet: miR-27b modulated the expression of Snail by inhibiting its degradation ( A , B ) The mRNA level of Snail in CFs treated with AngII combined with miR-27i ( A ) or miR2-7b ( B ). ( C ) The effect of miR-27b on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( D ) Roles of miR-27b in Snail ubiquitination. ( E ) Characterization of the messenger RNA (mRNA) of FBW7, which depicted miR-27b binding site (BS) in its 3′-untranslated region (UTR). ( F ) The interaction of FBW7 and Snail in CFs. ( G ) The effect of FBW7 overexpression on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( H ) The effect of FBW7 overexpression on the ubiquitination of Snail. Arrow indicates the GFP-FBW7. Data were represented as mean ± SEM (n=4). N, p >0.05.

    Techniques Used: Expressing, Ubiquitin Proteomics, Binding Assay, Over Expression

    FBW7 was the miR-27b target in cardiac fibrosis ( A – C ) The mRNA levels of FBW7 in serum-treated CFs ( A ), AngII ( B ), or miR-27b ( C ). ( D ) Snail expression in CFs transfection with miR-27b and/or peGFP-FBW7 plasmids. ( E , F ) The proliferation of CFs treated in ( D ) were analyzed by MTT test ( E ), and BrdU assay ( F ). ( G ) CFs transfected with miR-27i and/or FBW7 siRNA were subjected to AngII treatment as indicated. Snail expression was analyzed using WB. ( H , I ) The proliferation of CFs treated in ( G ) was analyzed by MTT test ( H ), and BrdU assay ( I ). Data were represented as mean ± SEM (n=4). *, p <0.05; **, p <0.01.
    Figure Legend Snippet: FBW7 was the miR-27b target in cardiac fibrosis ( A – C ) The mRNA levels of FBW7 in serum-treated CFs ( A ), AngII ( B ), or miR-27b ( C ). ( D ) Snail expression in CFs transfection with miR-27b and/or peGFP-FBW7 plasmids. ( E , F ) The proliferation of CFs treated in ( D ) were analyzed by MTT test ( E ), and BrdU assay ( F ). ( G ) CFs transfected with miR-27i and/or FBW7 siRNA were subjected to AngII treatment as indicated. Snail expression was analyzed using WB. ( H , I ) The proliferation of CFs treated in ( G ) was analyzed by MTT test ( H ), and BrdU assay ( I ). Data were represented as mean ± SEM (n=4). *, p <0.05; **, p <0.01.

    Techniques Used: Expressing, Transfection, BrdU Staining

    Antagomir-27b attenuated cardiac fibrosis in rat model of MI ( A ) Real-time PCR results of miR-27b levels in miR-27i or saline-treated specimens (3 weeks post-injection). ( B , C ) Analytical results of miR-27i-treated peri-infarct area of rat heart (3 weeks post-treatment). ( B ) Typical heart sections after treatments of Masson trichrome staining, laminin and collagen I immunostaining. Scale bar, 20 μm. ( C ) The percentage of tissue area represented the deposition of collagen I, where the automated image analyzer was used for its quantification. ( D ) Quantitative reverse transcription–PCR results of collagen I, collagen III, and MMP-9 mRNA levels. ( E ) FBW7 and Snail expression in rat heart. Data were represented as mean ± SEM (n=6). *, p <0.05; **, p <0.01.
    Figure Legend Snippet: Antagomir-27b attenuated cardiac fibrosis in rat model of MI ( A ) Real-time PCR results of miR-27b levels in miR-27i or saline-treated specimens (3 weeks post-injection). ( B , C ) Analytical results of miR-27i-treated peri-infarct area of rat heart (3 weeks post-treatment). ( B ) Typical heart sections after treatments of Masson trichrome staining, laminin and collagen I immunostaining. Scale bar, 20 μm. ( C ) The percentage of tissue area represented the deposition of collagen I, where the automated image analyzer was used for its quantification. ( D ) Quantitative reverse transcription–PCR results of collagen I, collagen III, and MMP-9 mRNA levels. ( E ) FBW7 and Snail expression in rat heart. Data were represented as mean ± SEM (n=6). *, p <0.05; **, p <0.01.

    Techniques Used: Real-time Polymerase Chain Reaction, Saline, Injection, Staining, Immunostaining, Reverse Transcription, Expressing

    Related Articles

    Luciferase:

    Article Title: MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway
    Article Snippet: An inverted microscope (Nikon Eclipse TS 100, Japan), was used for the observation of brown granules in the cells, with a random selection of 6 fields. .. The 3′UTR of the FBW7 luciferase reporter plasmid (pRL-TK 3′FBXW7 UTR) were obtained from Addgene (#26649). ..

    Plasmid Preparation:

    Article Title: MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway
    Article Snippet: An inverted microscope (Nikon Eclipse TS 100, Japan), was used for the observation of brown granules in the cells, with a random selection of 6 fields. .. The 3′UTR of the FBW7 luciferase reporter plasmid (pRL-TK 3′FBXW7 UTR) were obtained from Addgene (#26649). ..



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    fbw7 luciferase reporter plasmid  (Addgene inc)
    90
    Addgene inc fbw7 luciferase reporter plasmid
    miR-27b modulated the expression of Snail by inhibiting its degradation ( A , B ) The mRNA level of Snail in CFs treated with AngII combined with miR-27i ( A ) or miR2-7b ( B ). ( C ) The effect of miR-27b on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( D ) Roles of miR-27b in Snail ubiquitination. ( E ) Characterization of the messenger RNA (mRNA) of <t>FBW7,</t> which depicted miR-27b binding site (BS) in its 3′-untranslated region (UTR). ( F ) The interaction of FBW7 and Snail in CFs. ( G ) The effect of FBW7 overexpression on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( H ) The effect of FBW7 overexpression on the ubiquitination of Snail. Arrow indicates the GFP-FBW7. Data were represented as mean ± SEM (n=4). N, p >0.05.
    Fbw7 Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fbw7+luciferase+reporter+plasmid/pRL-TK+3'FBXW7+UTR+(Plasmid+%2326649)/pmc06949061-188-4-14
    Average 90 stars, based on 1 article reviews
    fbw7 luciferase reporter plasmid - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    miR-27b modulated the expression of Snail by inhibiting its degradation ( A , B ) The mRNA level of Snail in CFs treated with AngII combined with miR-27i ( A ) or miR2-7b ( B ). ( C ) The effect of miR-27b on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( D ) Roles of miR-27b in Snail ubiquitination. ( E ) Characterization of the messenger RNA (mRNA) of FBW7, which depicted miR-27b binding site (BS) in its 3′-untranslated region (UTR). ( F ) The interaction of FBW7 and Snail in CFs. ( G ) The effect of FBW7 overexpression on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( H ) The effect of FBW7 overexpression on the ubiquitination of Snail. Arrow indicates the GFP-FBW7. Data were represented as mean ± SEM (n=4). N, p >0.05.

    Journal: Aging (Albany NY)

    Article Title: MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway

    doi: 10.18632/aging.102465

    Figure Lengend Snippet: miR-27b modulated the expression of Snail by inhibiting its degradation ( A , B ) The mRNA level of Snail in CFs treated with AngII combined with miR-27i ( A ) or miR2-7b ( B ). ( C ) The effect of miR-27b on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( D ) Roles of miR-27b in Snail ubiquitination. ( E ) Characterization of the messenger RNA (mRNA) of FBW7, which depicted miR-27b binding site (BS) in its 3′-untranslated region (UTR). ( F ) The interaction of FBW7 and Snail in CFs. ( G ) The effect of FBW7 overexpression on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. ( H ) The effect of FBW7 overexpression on the ubiquitination of Snail. Arrow indicates the GFP-FBW7. Data were represented as mean ± SEM (n=4). N, p >0.05.

    Article Snippet: The 3′UTR of the FBW7 luciferase reporter plasmid (pRL-TK 3′FBXW7 UTR) were obtained from Addgene (#26649).

    Techniques: Expressing, Ubiquitin Proteomics, Binding Assay, Over Expression

    FBW7 was the miR-27b target in cardiac fibrosis ( A – C ) The mRNA levels of FBW7 in serum-treated CFs ( A ), AngII ( B ), or miR-27b ( C ). ( D ) Snail expression in CFs transfection with miR-27b and/or peGFP-FBW7 plasmids. ( E , F ) The proliferation of CFs treated in ( D ) were analyzed by MTT test ( E ), and BrdU assay ( F ). ( G ) CFs transfected with miR-27i and/or FBW7 siRNA were subjected to AngII treatment as indicated. Snail expression was analyzed using WB. ( H , I ) The proliferation of CFs treated in ( G ) was analyzed by MTT test ( H ), and BrdU assay ( I ). Data were represented as mean ± SEM (n=4). *, p <0.05; **, p <0.01.

    Journal: Aging (Albany NY)

    Article Title: MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway

    doi: 10.18632/aging.102465

    Figure Lengend Snippet: FBW7 was the miR-27b target in cardiac fibrosis ( A – C ) The mRNA levels of FBW7 in serum-treated CFs ( A ), AngII ( B ), or miR-27b ( C ). ( D ) Snail expression in CFs transfection with miR-27b and/or peGFP-FBW7 plasmids. ( E , F ) The proliferation of CFs treated in ( D ) were analyzed by MTT test ( E ), and BrdU assay ( F ). ( G ) CFs transfected with miR-27i and/or FBW7 siRNA were subjected to AngII treatment as indicated. Snail expression was analyzed using WB. ( H , I ) The proliferation of CFs treated in ( G ) was analyzed by MTT test ( H ), and BrdU assay ( I ). Data were represented as mean ± SEM (n=4). *, p <0.05; **, p <0.01.

    Article Snippet: The 3′UTR of the FBW7 luciferase reporter plasmid (pRL-TK 3′FBXW7 UTR) were obtained from Addgene (#26649).

    Techniques: Expressing, Transfection, BrdU Staining

    Antagomir-27b attenuated cardiac fibrosis in rat model of MI ( A ) Real-time PCR results of miR-27b levels in miR-27i or saline-treated specimens (3 weeks post-injection). ( B , C ) Analytical results of miR-27i-treated peri-infarct area of rat heart (3 weeks post-treatment). ( B ) Typical heart sections after treatments of Masson trichrome staining, laminin and collagen I immunostaining. Scale bar, 20 μm. ( C ) The percentage of tissue area represented the deposition of collagen I, where the automated image analyzer was used for its quantification. ( D ) Quantitative reverse transcription–PCR results of collagen I, collagen III, and MMP-9 mRNA levels. ( E ) FBW7 and Snail expression in rat heart. Data were represented as mean ± SEM (n=6). *, p <0.05; **, p <0.01.

    Journal: Aging (Albany NY)

    Article Title: MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway

    doi: 10.18632/aging.102465

    Figure Lengend Snippet: Antagomir-27b attenuated cardiac fibrosis in rat model of MI ( A ) Real-time PCR results of miR-27b levels in miR-27i or saline-treated specimens (3 weeks post-injection). ( B , C ) Analytical results of miR-27i-treated peri-infarct area of rat heart (3 weeks post-treatment). ( B ) Typical heart sections after treatments of Masson trichrome staining, laminin and collagen I immunostaining. Scale bar, 20 μm. ( C ) The percentage of tissue area represented the deposition of collagen I, where the automated image analyzer was used for its quantification. ( D ) Quantitative reverse transcription–PCR results of collagen I, collagen III, and MMP-9 mRNA levels. ( E ) FBW7 and Snail expression in rat heart. Data were represented as mean ± SEM (n=6). *, p <0.05; **, p <0.01.

    Article Snippet: The 3′UTR of the FBW7 luciferase reporter plasmid (pRL-TK 3′FBXW7 UTR) were obtained from Addgene (#26649).

    Techniques: Real-time Polymerase Chain Reaction, Saline, Injection, Staining, Immunostaining, Reverse Transcription, Expressing